师资队伍

乐易林

乐易林,男,博士,毕业于江南大学发酵工程专业,获工学博士学位。2010年至今在87978797威尼斯老品牌工作。

主要从事极端微生物在能源转化和环境修复中的应用等相关研究工作。利用合成生物学思路对极端微生物进行遗传改造,强化极端微生物的能源转化效率和环境修复效率。

相关研究成果以第一作者或通讯作者发表在《生物工程学报》、《微生物学通报》、《中国生物工程杂志》、Applied and Environmental Microbiology、Advances in Applied Microbiology、DNA research、Enzyme and Microbial Technology、Protein Expression and Purification、Fuel和Journal of Hazardous Materials等国内外期刊上。

1) Yilin Le*; Yu Fu; Jianzhong Sun*; Genome editing of the anaerobic thermophile Thermoanaerobacter ethanolicus using thermostable Cas9, Applied and Environmental Microbiology, 2021, 87(1): e01773-20.

2) Yilin Le*, Xing He, Jianzhong Sun*; Thermostable CRISPR/Cas9 genome editing system and its application in construction of cell factories with thermophilic bacteria: a review. Chinese Journal of Biotechnology. 2022, 38(4):1475-1489.

3) Yilin Le*; Jianzhong Sun*; CRISPR/Cas genome editing systems in thermophiles: current status, associated challenges, and future perspectives, Advances in Applied Microbiology, 2022, 118: 1-30.

4) Huilei Wang; Yilin Le*; Jianzhong Sun*; Consolidated bioprocessing of biomass and synthetic cadmium wastewater substrates for enhancing hydrogen production by Clostridium thermocellum-CdS complex, Fuel, 2022, 316: 123207.

5) Huilei Wang; Yilin Le*; Jianzhong Sun*; Light-driven bio-decolorization of triphenylmethane dyes by a Clostridium thermocellum-CdS biohybrid, Journal of Hazardous Materials, 2022, 431: 128596.

6) Yilin Le*, Huilei Wang. High-level soluble expression of a thermostable xylanase from thermophilic fungus Thermomyces lanuginosus in Escherichia coli via fusion with OsmY protein. Protein Expression and Purification, 2014, 99: 1-5.

7) Yilin Le, Huayou Chen, ROBERT Zagursky, J.H. DAVID Wu*, Weilan Shao*.­­ Thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR. DNA Research, 2013, 20(4):375-382.

8) Yilin Le, Jingjing Peng, Jianjun Pei, Huazhong Li, Zuoying Duan, Weilan Shao*. Properties of an NAD+-dependent DNA ligase from the hyperthermophile Thermotoga maritima and its application in PCR amplification of long DNA fragments. Enzyme And Microbial Technology, 2010, 46(2): 113-117.

9) Jianjun Pei, Qing Zhou, Yu Jiang, Yilin Le, Huazhong Li, Weilan Shao*, JuergenWiegel*. Thermoanaerobacter spp control ethanol pathway via transcriptional regulation and versatility of key enzymes. Metabolic Engineering, 2010, 12(5): 420-428.

发明专利:

1) 乐易林,孙建中,倪黎,一种酶法制备乙酰辅酶A的方法,专利号:ZL201810095650.7,授权公告日:2020年02月21日。

2) 乐易林,傅毓,孙建中,一种嗜热厌氧乙醇杆菌基因编辑的载体质粒及其应用,申请号:201911227684.8。